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p1 eyfp pa  (Addgene inc)


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    Structured Review

    Addgene inc p1 eyfp pa
    P1 Eyfp Pa, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p1+eyfp+pa/P1-EYFP-pA+(Construct+5)+(Plasmid+%2355197)/pm33930275__sb1c00037_si_001-143-41-42
    Average 90 stars, based on 3 article reviews
    p1 eyfp pa - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Sequencing:

    Article Title: High-Performance Allosteric Conditional Guide RNAs for Mammalian Cell-Selective Regulation of CRISPR/Cas.
    Article Snippet: S1.3.2 Cell culture and induction assay Mammalian cgRNA performance was assayed using a modified version of previously described fluorescent protein gene induction assays.12–14 Briefly, four plasmids (Figure S6 and Table S1) were co-transfected into HEK 293T cells and CRISPR/dCas9mediated fluorescent protein gene induction was assayed by flow cytometry after 24 h. The SP-dCas9-VPR plasmid was a gift from G. Church (Addgene plasmid #63798).15 The reporter plasmid P1-minCMVPhiYFP was synthesized and cloned by Twist Bioscience. .. The insert sequence was based on the reporter-gT1 plasmid (Addgene plasmid #47320)12 and was modified to replace the T1 gRNA target-binding region with a P1 gRNA target-binding region13 and the dTomato fluorescent protein was replaced with the PhiYFP fluorescent protein from P1-EYFP-pA (Addgene plasmid #55197).13 Target PAM ...... Plasmid 1: SP-dCas9–VPR Plasmid 2: P1-minCMV–PhiYFP Plasmid 3: cgRNA Plasmid 4: Trigger d e f u d* e* f* xrRNA Figure S6: Assay schematic. .. S10 Plasmid Function Fluorescent protein Parent plasmid SP-dCas9-VPR S. pyogenes dCas9 fused to VPR induction domains (CMV promoter) none Addgene #63798 P1-minCMV-PhiYFP P1 gRNA binding site upstream of a minimal CMV promoter expressing PhiYFP reporter PhiYFP Twist Amp High Copy, Addgene #47320 & #55197 cgRNA hU6 expression of a single cgRNA miRFP670 (pPGK) Addgene #49016 Trigger hU6 expression of a single trigger EBFP2 (pPGK) Addgene #49016 Table S1: HEK 293T induction assay components.

    Plasmid Preparation:

    Article Title: High-Performance Allosteric Conditional Guide RNAs for Mammalian Cell-Selective Regulation of CRISPR/Cas.
    Article Snippet: S1.3.2 Cell culture and induction assay Mammalian cgRNA performance was assayed using a modified version of previously described fluorescent protein gene induction assays.12–14 Briefly, four plasmids (Figure S6 and Table S1) were co-transfected into HEK 293T cells and CRISPR/dCas9mediated fluorescent protein gene induction was assayed by flow cytometry after 24 h. The SP-dCas9-VPR plasmid was a gift from G. Church (Addgene plasmid #63798).15 The reporter plasmid P1-minCMVPhiYFP was synthesized and cloned by Twist Bioscience. .. The insert sequence was based on the reporter-gT1 plasmid (Addgene plasmid #47320)12 and was modified to replace the T1 gRNA target-binding region with a P1 gRNA target-binding region13 and the dTomato fluorescent protein was replaced with the PhiYFP fluorescent protein from P1-EYFP-pA (Addgene plasmid #55197).13 Target PAM ...... Plasmid 1: SP-dCas9–VPR Plasmid 2: P1-minCMV–PhiYFP Plasmid 3: cgRNA Plasmid 4: Trigger d e f u d* e* f* xrRNA Figure S6: Assay schematic. .. S10 Plasmid Function Fluorescent protein Parent plasmid SP-dCas9-VPR S. pyogenes dCas9 fused to VPR induction domains (CMV promoter) none Addgene #63798 P1-minCMV-PhiYFP P1 gRNA binding site upstream of a minimal CMV promoter expressing PhiYFP reporter PhiYFP Twist Amp High Copy, Addgene #47320 & #55197 cgRNA hU6 expression of a single cgRNA miRFP670 (pPGK) Addgene #49016 Trigger hU6 expression of a single trigger EBFP2 (pPGK) Addgene #49016 Table S1: HEK 293T induction assay components.

    Modification:

    Article Title: High-Performance Allosteric Conditional Guide RNAs for Mammalian Cell-Selective Regulation of CRISPR/Cas.
    Article Snippet: S1.3.2 Cell culture and induction assay Mammalian cgRNA performance was assayed using a modified version of previously described fluorescent protein gene induction assays.12–14 Briefly, four plasmids (Figure S6 and Table S1) were co-transfected into HEK 293T cells and CRISPR/dCas9mediated fluorescent protein gene induction was assayed by flow cytometry after 24 h. The SP-dCas9-VPR plasmid was a gift from G. Church (Addgene plasmid #63798).15 The reporter plasmid P1-minCMVPhiYFP was synthesized and cloned by Twist Bioscience. .. The insert sequence was based on the reporter-gT1 plasmid (Addgene plasmid #47320)12 and was modified to replace the T1 gRNA target-binding region with a P1 gRNA target-binding region13 and the dTomato fluorescent protein was replaced with the PhiYFP fluorescent protein from P1-EYFP-pA (Addgene plasmid #55197).13 Target PAM ...... Plasmid 1: SP-dCas9–VPR Plasmid 2: P1-minCMV–PhiYFP Plasmid 3: cgRNA Plasmid 4: Trigger d e f u d* e* f* xrRNA Figure S6: Assay schematic. .. S10 Plasmid Function Fluorescent protein Parent plasmid SP-dCas9-VPR S. pyogenes dCas9 fused to VPR induction domains (CMV promoter) none Addgene #63798 P1-minCMV-PhiYFP P1 gRNA binding site upstream of a minimal CMV promoter expressing PhiYFP reporter PhiYFP Twist Amp High Copy, Addgene #47320 & #55197 cgRNA hU6 expression of a single cgRNA miRFP670 (pPGK) Addgene #49016 Trigger hU6 expression of a single trigger EBFP2 (pPGK) Addgene #49016 Table S1: HEK 293T induction assay components.



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