p1 eyfp pa (Addgene inc)
90
Structured Review
Addgene inc
p1 eyfp pa
P1 Eyfp Pa, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p1+eyfp+pa/P1-EYFP-pA+(Construct+5)+(Plasmid+%2355197)/pm33930275__sb1c00037_si_001-143-41-42
Average 90 stars, based on 3 article reviews
P1 Eyfp Pa, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p1+eyfp+pa/P1-EYFP-pA+(Construct+5)+(Plasmid+%2355197)/pm33930275__sb1c00037_si_001-143-41-42
Average 90 stars, based on 3 article reviews
p1 eyfp pa - by Bioz Stars,
2026-09
90/100 stars
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Sequencing:Article Title: High-Performance Allosteric Conditional Guide RNAs for Mammalian Cell-Selective Regulation of CRISPR/Cas. Article Snippet: S1.3.2 Cell culture and induction assay Mammalian cgRNA performance was assayed using a modified version of previously described fluorescent protein gene induction assays.12–14 Briefly, four plasmids (Figure S6 and Table S1) were co-transfected into HEK 293T cells and CRISPR/dCas9mediated fluorescent protein gene induction was assayed by flow cytometry after 24 h. The SP-dCas9-VPR plasmid was a gift from G. Church (Addgene plasmid #63798).15 The reporter plasmid P1-minCMVPhiYFP was synthesized and cloned by Twist Bioscience. .. The insert sequence was based on the reporter-gT1 plasmid (Addgene plasmid #47320)12 and was modified to replace the T1 gRNA target-binding region with a P1 gRNA target-binding region13 and the dTomato fluorescent protein was replaced with the PhiYFP fluorescent protein from Plasmid Preparation:Article Title: High-Performance Allosteric Conditional Guide RNAs for Mammalian Cell-Selective Regulation of CRISPR/Cas. Article Snippet: S1.3.2 Cell culture and induction assay Mammalian cgRNA performance was assayed using a modified version of previously described fluorescent protein gene induction assays.12–14 Briefly, four plasmids (Figure S6 and Table S1) were co-transfected into HEK 293T cells and CRISPR/dCas9mediated fluorescent protein gene induction was assayed by flow cytometry after 24 h. The SP-dCas9-VPR plasmid was a gift from G. Church (Addgene plasmid #63798).15 The reporter plasmid P1-minCMVPhiYFP was synthesized and cloned by Twist Bioscience. .. The insert sequence was based on the reporter-gT1 plasmid (Addgene plasmid #47320)12 and was modified to replace the T1 gRNA target-binding region with a P1 gRNA target-binding region13 and the dTomato fluorescent protein was replaced with the PhiYFP fluorescent protein from Modification:Article Title: High-Performance Allosteric Conditional Guide RNAs for Mammalian Cell-Selective Regulation of CRISPR/Cas. Article Snippet: S1.3.2 Cell culture and induction assay Mammalian cgRNA performance was assayed using a modified version of previously described fluorescent protein gene induction assays.12–14 Briefly, four plasmids (Figure S6 and Table S1) were co-transfected into HEK 293T cells and CRISPR/dCas9mediated fluorescent protein gene induction was assayed by flow cytometry after 24 h. The SP-dCas9-VPR plasmid was a gift from G. Church (Addgene plasmid #63798).15 The reporter plasmid P1-minCMVPhiYFP was synthesized and cloned by Twist Bioscience. .. The insert sequence was based on the reporter-gT1 plasmid (Addgene plasmid #47320)12 and was modified to replace the T1 gRNA target-binding region with a P1 gRNA target-binding region13 and the dTomato fluorescent protein was replaced with the PhiYFP fluorescent protein from |